Services

Karyotyping

Name, Description Units PHS Fee DFCI Fee Non-PHS Academic Fee Non-PHS Industry Fee
Chromosome analysis Complex (human and mouse)

Standard metaphase chromosome analysis, including 20 cells counted and minimum 5 karyotypes prepared.

Each $916.00 $916.00 $1,320.00 $1,457.00
Chromosome Harvest (no analysis)

Cytogenetic harvest for metaphases spreads, checked for quality and either slides or pellets returned to investigator.

Each $144.00 $144.00 $208.00 $229.00
Chromosome analysis Simple (human and mouse)

Standard metaphase chromosome analysis, including 20 cells counted and minimum 3-5 karyotypes prepared.

Each $577.00 $577.00 $831.00 $918.00
Partial Chromosome Analysis

Metaphase chromosome analysis, including up to 10 cells counted and 1-2 karyotypes prepared when insufficient analyzable cells are available for standard analysis.

Each $394.00 $394.00 $568.00 $627.00
Quick Scan (human and mouse)

Metaphase chromosome analysis, including 10 cells counted and analyzed at scope only; no karyotypes.

Each $210.00 $210.00 $303.00 $334.00
Unsuccessful Chromosome Study

A reduced charge if multiple attempts to harvest a specimen are made without obtaining analyzable metaphases.

Each $191.00 $191.00 $276.00 $304.00

FISH

Name, Description Units PHS Fee DFCI Fee Non-PHS Academic Fee Non-PHS Industry Fee
Interphase FISH

Fluorescent In Situ Hybridization scored on interphase cells (nuclei) for signal copy number, with commercial or custom- made probe (additional charges for preparing probes may apply, see below). Charge is for 1 slide with 1 probe; additional probes and additional slides (up to 5 per batch) are charged as below.

Each (1st slide of each batch of $324.00 $324.00 $467.00 $516.00
Interphase Tissue Microarray (TMA) FISH

Fluorescent In Situ Hybridization scored on interphase cells (nuclei) in formalin-fixed paraffin-embedded tissue sections for signal copy number, with commercial or custom- made probe (additional charges for preparing probes may apply, see below). Charge is for hybridization of 1 slide; hourly rate for technical support (see below) is additional for scoring and imaging cores.

Each $2,743.00 $2,743.00 $3,950.00 $4,362.00
Metaphase FISH

Fluorescent In Situ Hybridization scored on chromosome spreads for signal location, with commercial or custom- made probe (additional charges for preparing probes may apply, see below). Charge is for 1 slide with 1 probe; additional probes and additional slides (up to 5 per batch) are charged as below. Cytogenetic harvest for metaphase chromosomes is included.

each (1st slide of each batch of $693.00 $693.00 $998.00 $1,102.00
Additional probe

Charge for a second (or third) probe run simultaneously on Metaphase, Interphase or Paraffin FISH slides.

1 $112.00 $112.00 $162.00 $179.00
Additional slide for FISH hybridization

Metaphase, Interphase or Paraffin FISH are run in batches; this reduced charge is for slides #2 through #5 of a 5 slide batch, run at the same time with the same probes.

Each (slides 2-5 of every batch $245.00 $245.00 $353.00 $390.00
M-band FISH

Multicolor labeling of a single human chromosome, performed on metaphase spreads.

Each $1,021.00 $1,021.00 $1,471.00 $1,624.00
Multi-Plex FISH Complex (M-FISH)

MFISH or SKY for human or mouse with “paint” probes differentially labeled for each chromosome, performed on metaphase spreads.

Each $3,086.00 $3,086.00 $4,444.00 $4,907.00
Multi-Plex FISH Simple (M-FISH)

MFISH or SKY for human or mouse with “paint” probes differentially labeled for each chromosome, performed on metaphase spreads.

Each $1,437.00 $1,437.00 $2,070.00 $2,285.00
Paraffin FISH

Fluorescent In Situ Hybridization scored on interphase cells (nuclei) in formalin-fixed paraffin-embedded tissue sections for signal copy number, with commercial or custom- made probe (additional charges for preparing probes may apply, see below). Charge is for 1 slide with 1 probe; additional probes and additional slides (up to 5 per batch) are charged as below.

each (1st slide of each batch of $607.00 $607.00 $875.00 $966.00
Unsuccessful FISH Study

A reduced charge if multiple attempts to hybridize an investigator-provided sample are made without obtaining analyzable signals.

Each $210.00 $210.00 $303.00 $334.00

DNA

Name, Description Units PHS Fee DFCI Fee Non-PHS Academic Fee Non-PHS Industry Fee
BAC/Fosmid DNA preparation for FISH

Growing and extracting DNA for FISH probe from BAC, PAC, cosmid, fosmid, or plasmid.

Each $192.00 $192.00 $277.00 $306.00
DNA labeling

Labeling of Core-provided or investigator-provided DNA for FISH probe.

Each $158.00 $158.00 $228.00 $252.00
Genomic DNA isolation (blood, cells, etc)

Isolation of DNA from blood or other tissues.

Each, per slide $112.00 $112.00 $162.00 $179.00
Purchase of BAC or fosmid (clones)

Obtaining BAC, PAC, cosmid or fosmid probes for human or mouse FISH.

Each $171.00 $171.00 $247.00 $272.00

Array

Name, Description Units PHS Fee DFCI Fee Non-PHS Academic Fee Non-PHS Industry Fee
Agilent aCGH (180K/244K/1M) (human, mouse)

Array Comparative Genomic Hybridization for human or mouse using commercial Agilent 180K/244K/1M array

Each $1,604.00 $1,604.00 $2,310.00 $2,551.00

Miscellaneous

Name, Description Units PHS Fee DFCI Fee Non-PHS Academic Fee Non-PHS Industry Fee
Culture initiation, maintenance and freezing

Initiation of cell cultures from skin or other tissue, including expansion and freezing in liquid nitrogen

Each $250.00 $250.00 $360.00 $398.00
Technical cytogenetic support

An hourly rate for additional scoring or other specific technical support, including BioView scans.

Hour $48.00 $48.00 $70.00 $77.00

Task

Name, Description Units PHS Fee DFCI Fee Non-PHS Academic Fee Non-PHS Industry Fee
Additional probe

Charge for a second (or third) probe run simultaneously on Metaphase, Interphase or Paraffin FISH slides.

$0.00 $0.00 $0.00 $0.00