About Us

BWH Aquatics Facility at BWH is a newly designed 5,000+ fish tank facility, located in Eugene Braunwald Research Center at 221 Longwood Ave. It is a state-of-the-art 4000 sqft facility built by Tecniplast in 2012. The facility is equipped with 91 racks holding standard and nursery/isolation zebrafish tanks, 9 large scale breeding chambers (ispawns) and a quarantine space with two stand-alone housing systems. Wild-type lines are maintained in-house for shared use. Wet lab space is available for breeding and line maintenance and there is a dedicated procedure room for embryo manipulation and genotyping.  Full time staff performs daily feeding (including weekends) and maintenance and is available for assistance for procedure training and general husbandry questions. All BWH Zebrafish Core users must be associated with an IACUC-approved animal protocol.

Location of Core:

The facility is located at 221 Longwood Ave, B-033

Our team:

Administrative Director: Angela Vail

Phone: (617) 525-8829
Email: avail3@partners.org

Scientific Director: Wolfram Goessling, M.D., Ph.D

Phone: (617) 632-3474
Email: WGoessling@partners.org

Facility and Project Manager: Eva Buys, Ph.D

Phone: (617) 732-8578
Email: eplovie@partners.org

Technicians:

Thomas Pedulla

Phone: (617) 732-8578
Email: tpedulla@partners.org

Alex Cintolo (weekends)

Phone: (617) 732-8578
Email:acintolo@partners.org   

For more information and to get access, please contact Eva Buys at eplovie@partners.org or at 617-732-8578

Offered Services:

  • Housing, feeding and general care of Zebrafish (feeding, health monitoring, system maintenance)
  • Propagation of wild-type Zebrafish stocks for embryo production
  • Training in Zebrafish handling, husbandry and microinjection (for morpholino’s or transgenesis) and imaging
  • Assistance with Zebrafish protocol planning and preparation
  • Shipping and receiving live Zebrafish
  • sperm freezing and in vitro fertilization (pending)
  • Study Consultation


Facilities and Equipment:


*Aquaculture Equipment:

  • 79 racks holding up to 50 standard sized adult zebrafish tanks (3.3 and 8L)
  • 12 racks for larval fish or isolation type experiments (1.1L)
  • 9 large scale breeding chambers (ispawns)
  • separate quarantine space with two stand-alone housing systems
  • food preparation room
  • dishwashers are located throughout the whole facility as well as a dedicated dishwashing room

*Laboratory Equipment:

  • Three large and two small Thermo Scientific Heratherm incubators to enable embryo maintenance at variable temperatures.
  • Two under counter Refrigerators, four under counter -20 freezers and a -80 freezer for the storage of laboratory and tissue samples
  • A thermoscientific Locator 6PLUS cryogenic storage vessel to store sperm samples.
  • Eight injection stations, each equipped with an Eppendorf Femtojet Micro injection system and a Nikon SMZ800 stereomicroscope and a Sutter Instrument Model P-1000 micropipette needle puller.
  • A Bio-Rad T100 Thermal Cycler PCR machine for genotyping.
  • A dedicated microscope screening room with a Nikon fluorescence stereomicroscope SMZ1500 and a Nikon eclipse Ni high magnification microscope, each equipped with NIS-Elements microscope imaging Software.

Getting started:

ALL users must…

  • Be listed on an IACUC-approved animal protocol (for Zebrafish)
  • Undergo training on BWH ARF Core Facility rules and standard operations prior to gaining card access
  • Perform  AALAS learning library online Training as appropriate to Zebrafish use
  • Have BWH Occupational Health Clearances to work with animals
  • Work humanely with experimental Zebrafish lines, following IACUC approved standard procedures for housing densities and tank maintenance.

Fee Structure:

Rack Fee (includes 1 rack holding up 50 3.3L tanks or 25 8 liter tanks and use of all nursery/isolation rack space and equipment listed above)

$3240/year -$270/month (partners)

$7460.77/year-$622/month (non-partners)


Revised on 2018-10-15 18:57:49 UTC