MGH Cancer Center Imaging Core

The Imaging Core provides access to a Zeiss LSM710 laser scanning confocal microscope, Leica Aperio CSO digital pathology slide scanner, Akoya Polaris and Vectra multispectral slide scanners,  and also provides services for biomarker staining and image quantification.  The Imaging Core is a limited to Cancer Center and Molecular Pathology members only.  

Imagers

Trained users have 24/7 scheduled access to imagers.  Assisted imaging is available upon request for an additional fee.

Zeiss LSM710 Laser Scanning Confocal

Laser scanning confocal microscope capable of capturing high resolution images with z-stacks on fixed of live samples using 6 different laser lines (405, 458, 488, 514, 561, 633 nm). 

  • Multi-position imaging
  • Time-lapse imaging
  • Live cell imaging with incubation and CO2

Leica Aperio CSO Digital Pathology Scanner

Automated pathology microscope scanner ideal for scanning IHC tissue slides, with capability of scanning up to 5 slides in a single set up.

  • Choose from dry scanning 20x or 40x
  • Superb image quality for publication or downstream image quantification
  • Ideal for IHC FFPE tissue sections or hematology and cytology slides

Akoya Polaris Slide Scanner 

Automated multispectral fluorescence slide scanner capable of 20x and 40x resolution images as well as bright field imaging and  standard fluorescence scans.  Ideal for:

  • Multiplexed stains
  • Samples with high confounding autofluorescence (like FFPE specimens)

Akoya Vectra slide scanner 

Multispectral fluorescence slide scanner capable of 10x and 20x resolution images.  


Image quantification

Computational analysis of images is performed using high-end software (Halo by Indica Labs or VIS by Visiopharm).  Software is capable of cell segmentation using propriety methods or other AI (artificial intelligence) approaches, cell enumeration, RNA ISH enumeration, spatial profiling, heatmap generation, tissue registration, and more.  All analysis apps are tailored to each research project and output can be further analyzed using custom R or Python scripts.  Trained users have 24/7 scheduled access to analysis workstations. Consultation with Dr. Nieman required.


Biomarker Staining

Slide staining will be billed per slide and have a two-tiered structure dependent on whether reagents are provided by investigators.  All staining, with the exception of DNA fusion, will be performed on a Leica Bond RX autostainer to facilitate high-volume consistent stains.  Scope of work will be outlined during required consultation sessions.

Types of biomarker staining include:  multiplex protein fluorescence (FL) staining using Akoya Opal fluorophores, multiplex FL using ACD RNAscope ISH probes with Akoya Opal fluorophores, multiplex chromogenic RNAscope ISH, and accompanying singleplex and duplex IHC needed for optimization of multiplex staining, 

Publications

Please cite MGH Cancer Center / Molecular Pathology Imaging Core in the Acknowledgements section.

Contact

For consultation, please contact Linda Nieman at lnieman@mgh.harvard.edu and

For training requests, please contact Katherine Xu at khxu@mgh.harvard.edu