Center for Cellular Profiling: Cytometry and Single-Cell Multi-Omics Core

WEBSITE: https://ccp.bwh.harvard.edu/


Flow Cytometry Services


WEBSITE: https://ccp.bwh.harvard.edu/flow-cytometry-services/

INSTRUMENTS:

4-Laser BD FACSAria Fusion (16 fluorescent channels)* 

  • 405nm - 85mW - "Violet laser"; 488nm - 50mW - "Blue laser"; 561nm - 50mW - "Yellow/Green laser"; 640nm - 100mW - "Red laser"

5-Laser BD FACSAria Fusion (18 fluorescent channels)*

  • 355nm - 15mW - "UV laser"; 405nm - 85mW - "Violet laser"; 488nm - 50mW - "Blue laser"; 561nm - 50mW - "Yellow/Green laser"; 640nm - 100mW - "Red laser"

*Please review specific filter layouts by clicking links to respective 4-Laser vs 5-Laser instruments!

SONY ID7000 Spectral Analyzer  (5 lasers, 147 PMTs)

Single-Cell Multi-Omics Services


WEBSITE: https://ccp.bwh.harvard.edu/single-cell-services/

SERVICES: (MGB Academic rates shown**)

  • Single-Cell RNA-Sequencing 
  • Single-Cell Multiome Sequencing 
  • Single-Cell ATAC-Sequencing 
  • Single-Cell Flex Assay (Individual; set of 4; set of 16)
**Non-MGB Academic Institutions (44%) and For-Profit Industry (59%) will be charged IDCs on core services by MGB**


Spatial Transcriptomic Services


WEBSITE: https://ccp.bwh.harvard.edu/spatial-transcriptomics-services/

SERVICES: (MGB Academic rates shown**)

  • Xenium In Situ (Regular or 5K)
  • Visium HD 
  • Visium CytAssist (6.5mm*6.5mm or11mm*11mm)

ADD-ON SERVICES: 

  • Multimodal segmentation 
**Non-MGB Academic Institutions (44%) and For-Profit Industry (59%) will be charged IDCs on core services by MGB**


How to request a service


TO REQUEST CELL SORTING :

INTERNAL MGB-

  • Determine which instrument (4L vs 5L) to use based on excitation lasers needed
  • Check the schedule for open time (Monday – Friday, 9:30am-5pm) by following links on the menu to the left
  • Fill out a Sort Request Form
  • Send completed Sort Request Form to FACS@bwh.harvard.edu
  • Await response and confirmation of booking
NOTE: 24-hour cancellation policy- If a user cancels within 24-hours of their sort, we must charge for the time booked unless a last minute fill-in is possible


TO REQUEST SINGLE-CELL SEQUENCING :

NEW USER or NEW PROJECT QUESTIONNAIRE (required by all new users or new project requests): Please create a Power Point slide or two addressing the following questions found in this document: New User or New Project Introductory Questionnaire . For Single-Cell Flex Projects, please fill out this document: Single-Cell Flex New Project Questionnaire. 

INTERNAL MGB-

  • Create (or log in) an account on the Partners Core Management System
  • Fill out a Sequencing Request Form
  • Send completed Sequencing Request Form to SINGLECELL@bwh.harvard.edu
  • Once received a core representative will reach out to schedule sample submission


TO REQUEST SPATIAL TRANSCRIPTOMIC SERVICES :

NEW USER or NEW PROJECT QUESTIONNAIRE (required by all new users or new project requests): Please create a Power Point slide or two addressing the following questions found in this document: Spatial New Project Introductory Questionnaire

INTERNAL MGB-


EXTERNAL MGB-

  • When creating new account use this document for instructions: External MGB CMS Registration.pdf
  • PO information (make payable to): Mass General Brigham – Cores Facilities Office, 399 Revolution Drive, Suite 750, Somerville, MA 02145


If you have any questions, please contact either FACS@bwh.harvard.edu or SINGLECELL@bwh.harvard.edu