- Center for Cellular Profiling: Cytometry and Single Cell Multi-Omics Core
Welcome to the Center for Cellular Profiling: Cytometry and Single-Cell Multi-Omics Core
(formerly the Human Immunology Center Flow Cytometry Core & Single Cell Genomics Core)
Flow Cytometry Services
WEBSITE: https://ccp.bwh.harvard.edu/flow-cytometry-services/
ASSISTED CELL SORTING FEES:
- MGB Academic - $110/hour
- Non-MGB Academic - $159/hour
- For-Profit Industry/Biotech - $200/hour
INSTRUMENTS:
4-Laser BD FACSAria Fusion (16 fluorescent channels)*
- 405nm - 85mW - "Violet laser"
- 488nm - 50mW - "Blue laser"
- 561nm - 50mW - "Yellow/Green laser"
- 640nm - 100mW - "Red laser"
5-Laser BD FACSAria Fusion (18 fluorescent channels)*
- 355nm - 15mW - "UV laser"
- 405nm - 85mW - "Violet laser"
- 488nm - 50mW - "Blue laser"
- 561nm - 50mW - "Yellow/Green laser"
- 640nm - 100mW - "Red laser"
*Please review specific filter layouts by clicking links to respective 4-Laser vs 5-Laser instruments!
Single-Cell Multi-Omics Services
WEBSITE: https://ccp.bwh.harvard.edu/single-cell-services/
SERVICES/FEES: (MGB Academic rates shown**)
NOTE: New pricing will become effective starting on 3/1/2023.
- Single-Cell RNA-Sequencing - $4,400 per sample
- Single-Cell RNA-Sequencing (>10 Samples) - $3700 per sample
- Single-Cell Multiome Sequencing - $7700 per sample
- Single-Cell ATAC-Sequencing - $4400 per sample
- Single-Cell FFPE service - $5300 per sample
ADD-ON SERVICES: (rates are added to Single-Cell Sequencing price)
- CITEseq (Custom panel) - $1000 per sample
- CITEseq (Universal panel) - $1400 per sample
- Cell Hashing - $2200 per sample
- Immune Cell Repertoire - $1100 per sample
- Deep Sequencing - $1000 per sample
How to request a service
TO REQUEST CELL SORTING :
INTERNAL MGB-
- Determine which instrument (4L vs 5L) to use based on excitation lasers needed
- Check the schedule for open time (Monday – Friday, 9:30am-5pm) by following links on the menu to the left
- Fill out a Sort Request Form
- Send completed Sort Request Form to FACS@bwh.harvard.edu
- Await response and confirmation of booking
EXTERNAL MGB-
- When creating new account use this document for instructions: External MGB CMS Registration.pdf
- PO information (make payable to): Mass General Brigham – Cores Facilities Office, 399 Revolution Drive, Suite 750, Somerville, MA 02145
TO REQUEST SINGLE-CELL SEQUENCING :
NEW USER or NEW PROJECT QUESTIONNAIRE (required by all new users or new project requests): Please create a Power Point slide or two addressing the following questions found in this document: New User or New Project Introductory Questionnaire
INTERNAL MGB-
- Create (or log in) an account on the Partners Core Management System
- Fill out a Sequencing Request Form
- Send completed Sequencing Request Form to SINGLECELL@bwh.harvard.edu
- Once received a core representative will reach out to schedule sample submission
- When creating new account use this document for instructions: External MGB CMS Registration.pdf
- PO information (make payable to): Mass General Brigham – Cores Facilities Office, 399 Revolution Drive, Suite 750, Somerville, MA 02145