- Center for Cellular Profiling: Cytometry and Single Cell Multi-Omics Core
Center for Cellular Profiling: Cytometry and Single-Cell Multi-Omics Core
WEBSITE: https://ccp.bwh.harvard.edu/
Flow Cytometry Services
**Non-MGB Academic Institutions (44%) and For-Profit Industry (59%) will be charged IDCs on core services by MGB**
FACS Sorters :
4-Laser BD FACSAria Fusion (16 fluorescent channels)*
- 405nm - "Violet laser"; 488nm - "Blue laser"; 561nm - "Yellow/Green laser"; 640nm - "Red laser"
5-Laser BD FACSAria Fusion (18 fluorescent channels)*
- 355nm - "UV laser"; 405nm - "Violet laser"; 488nm - "Blue laser"; 561nm - "Yellow/Green laser"; 640nm - "Red laser"
Assisted sorting: $130/hour; Self sorting: $88/hour
FACS Analyzers:
SONY ID7000 Spectral Analyzer (5 lasers, 147 PMTs): $70/hour
BD Fortessa (5 lasers): $40/hour
BD Canto (3 laser): $37/hour
Single-Cell Multi-Omics Services
**Non-MGB Academic Institutions (44%) and For-Profit Industry (59%) will be charged IDCs on core services by MGB**
SERVICES: (MGB Academic rates shown**)
- Single-Cell RNA-Sequencing: $4,800/sample
- Single-Cell On-Chip Multiplexing Sequencing: $4,800/sample
- Single-Cell Multiome Sequencing: $8,100/sample
- Single-Cell ATAC-Sequencing: $4,800/sample
- Single-Cell Flex Assay (V1-up to 16-plex; V2-up to 96-plex): pricingi varies on numbers of samples
ADD-ON SERVICES:
- Cell-Hashing: $2,200/sample
- CITE-seq (custom or universal panel): $1,400-$2,200/sample
- Immune Cell Repertoire: $1,100/sample
Spatial Transcriptomic Services
**Non-MGB Academic Institutions (44%) and For-Profit Industry (59%) will be charged IDCs on core services by MGB**
SERVICES: (MGB Academic rates shown**)
- Xenium In Situ (Regular): $7,500/slide
- Xenium In Situ (5K): $12,000/slide
- Visium HD (6.5mm*6.5mm or11mm*11mm): $9,200/slide-$12,500/slide (Summer Promotion!)
- Visium CytAssist (6.5mm*6.5mm or11mm*11mm): $14,000/slide
ADD-ON SERVICES:
- Multimodal segmentation (Xenium Regular): $1,250/slide
- Protein Plex (Xenium Regular)
- RNA QC: $250 per up to 8 samples
How to request a service
TO REQUEST CELL SORTING :
- Check the schedule for open time (Monday – Friday, 9:30am-5pm) by following links on the menu to the left
- Fill out a Sort Request Form
- Send completed Sort Request Form to FACS@bwh.harvard.edu
- Await response and confirmation of booking
TO REQUEST SINGLE-CELL SEQUENCING :
NEW USER or NEW PROJECT QUESTIONNAIRE (required by all new users or new project requests): Please create a Power Point slide or two addressing the following questions found in this document: New User or New Project Introductory Questionnaire . For Single-Cell Flex Projects, please fill out this document: Single-Cell Flex New Project Questionnaire.
- Fill out a Sequencing Request Form
- Send completed Sequencing Request Form to SINGLECELL@bwh.harvard.edu
- Once received a core representative will reach out to schedule sample submission
TO REQUEST SPATIAL TRANSCRIPTOMIC SERVICES :
NEW USER or NEW PROJECT QUESTIONNAIRE (required by all new users or new project requests): Please create a Power Point slide or two addressing the following questions found in this document: Spatial New Project Introductory Questionnaire
- Fill out a Spatial Sequencing Request Form
- Send completed Sequencing Request Form to SINGLECELL@bwh.harvard.edu
- Once received a core representative will reach out to schedule sample submission
- When creating new account use this document for instructions: External MGB CMS Registration.pdf
- PO information (make payable to): Mass General Brigham – Cores Facilities Office, 399 Revolution Drive, Suite 750, Somerville, MA 02145
If you have any questions, please contact either FACS@bwh.harvard.edu or SINGLECELL@bwh.harvard.edu
