The CND Flow Cytometry Core Facility

The ARCND Flow Cytometry Core Facility is a multi-purpose, multi-instrument, multi-optional cell sorting and analyzing facility.  We have 3 - BD FACSAria cell sorters and 3 multi-laser analyzers.  Each sorter can sort cells based on  11  or 15 parameters, and sort the desired populations at one cell per well into plates or up to 4 tubes simultaneously.  We have 3 analyzers ranging from a 3 laser - 8 color LSRII to  5 laser - 18 color BD Fortessa and 29 color BD A5 FACS Symphony.

Sorters
A - BD FACS Standard Aria IIIu      -> 4 lasers : Blue->2 colors, FSC & SSC/ YG->4 colors/ Red->3 colors/ Violet->6 colors (15 color parameters)

B - BD FACS StandardAria IIIu       -> 4 lasers : Blue->2 colors, FSC & SSC/ YG->4 colors/ Red->3 colors/ Violet->6 colors (15 color parameters)

C - BD FACSAria SORP                  -> 3 lasers : Blue->5 colors, FSC & SSC/Red->3 colors/ Ultra Violet->3 colors (11 color parameters)  

Cytek® Aurora CS                        -> 5 Lasers:  Blue, YellowGreen, Red, Violet, UltraViolet (40+ color FSC and SSC B & V)

Analyzers
BD Facs Symphony A5                  -> 5 lasers : Blue->6 colors, FSC & SSC/ YG->5 colors/ Red->3 colors/ Violet->8 colors/ Ultra Violet->7 colors (29 color parameters)

BD Fortessa                                   -> 5 lasers : Blue->2 colors, FSC & SSC/ YG->5 colors/ Red->3 colors/ Violet->6 colors/ Ultra Violet-> 2 colors (18 color parameters)

A-Cytek® Aurora                         -> 5 Lasers:  Blue, YellowGreen, Red, Violet, UltraViolet (40+ color FSC and SSC B & V)

B-Cytek® Aurora                         -> 5 Lasers:  Blue, YellowGreen, Red, Violet, UltraViolet (40+ color FSC and SSC B & V)







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