- The Center for Neurologic Diseases (ARCND) Flow Cytometry Core Facility
The CND Flow Cytometry Core Facility
The ARCND Flow Cytometry Core Facility is a multi-purpose, multi-instrument, multi-optional cell sorting and analyzing facility. We have 3 - BD FACSAria cell sorters and 3 multi-laser analyzers. Each sorter can sort cells based on 11 or 15 parameters, and sort the desired populations at one cell per well into plates or up-to 4 tubes simultaneously. We have 3 analyzers ranging from a 3 laser - 8 color LSRII to 5 laser - 18 color BD Fortessa and 29 color BD A5 FACS Symphony.
A - BD FACS Standard Aria IIu -> 4 lasers : Blue->2 colors, FSC & SSC/ YG->4 colors/ Red->3 colors/ Violet->6 colors (15 color parameters)
B - BD FACS StandardAria IIu -> 4 lasers : Blue->2 colors, FSC & SSC/ YG->4 colors/ Red->3 colors/ Violet->6 colors (15 color parameters)
C - BD FACSAria SORP -> 3 lasers : Blue->5 colors, FSC & SSC/Red->3 colors/ Ultra Violet->3 colors (11 color parameters)
BD Facs Symphony A5 -> 5 lasers : Blue->6 colors, FSC & SSC/ YG->5 colors/ Red->3 colors/ Violet->8 colors/ Ultra Violet->7 colors (29 color parameters)
BD Fortessa -> 5 lasers : Blue->2 colors, FSC & SSC/ YG->5 colors/ Red->3 colors/ Violet->6 colors/ Ultra Violet-> 2 colors (18 color parameters)
BD LSRII -> 3 lasers : Blue->4 colors, FSC & SSC/ Red->2 colors/ Violet->2 colors (8 color parameters)
