Welcome to the HSCI-CRM Flow Cytometry Core Facility!


The CRM Flow Cytometry Core Facility offers dedicated research space, specialized equipment, and trained technicians to assist researchers with their cytometry sorting and analysis needs.

Hours of Operation: Monday-Thursday 10:30AM-7PM, Friday 8:30am-7pm
Staff: Maris Handley, Gloria Lima, and Jacqueline Choi

Email: crm.flow.cytometry@gmail.com

Friend us on Facebook! (Crm Flow Cytometry Facility)

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We’d like to ask your help in having a better understanding of sorting efficiency, and which cell types are most affected. If you have enough cells to check, and a method to count them, we’d appreciate it if you would let us know your counts post-sort. This information will help us advise other users and troubleshoot should issues come up.

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Self-run time opens for Aria 2 and 3, and the Hulk (Quant VYB) starting on July 6th!

Staff will be going back to our “normal” schedule as of July 16th

Aria 1 will be coming back online for staff operation. Self-run on the Arias will still be available for only Aria 2 and 3 to help maintain distancing. When policies change, we will add time on Aria 1.

On the self-run machines, there will still be a limit of one person at the machine at a time. We tried occasionally having 2 people at the LSRII as long as there was some distancing, but this isn’t working. Having an additional person in the facility causes other issues since only a certain number of people can be in the room at a time. When you use a cytometer, please be aware of other people in the room, follow all cleaning procedures for benches and keyboards and mice…and your hands! Be sure to wash your hands before you leave! 

6ft of distance will still need to be maintained between people, so you will still need to drop Aria sorts/samples outside on the bench, with a completed drop off form. If you prefer, you can email it to us ahead of your appointment. Try to bring all of your controls and samples at the same time, and pick up your samples/sorted cells when your appointment is done. We don’t have enough space on the bench to allow racks/ice buckets to be left for longer periods of time.

6ft of distance also means that training of any kind is still not possible.  

We’ve received masks and gowns and can now continue with BSL2+ sorting. If working with primary cells, be sure you have reviewed your biosafety protocol and that the Biosafety Office still approves sorting (aerosolizing!) your cells given the risk of COVID19 being present in the tissue/cells you use.

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New? Start here:

Biosafety Agreement – For all new users. Please complete this form if you intend to use our core facility. This needs to be submitted to us before scheduling any time.

Administrative and Experimental Info Sheet — This sheet will provide you with all of the necessary information you will need prior to using our core. 
We are happy to answer any questions you may have!

Pricing information for each of our services and equipment can be found here

Interested in training on one of our machines? Click here


We now offer BSL2+ sorting!

We are now able to run a wider variety of BSL2 samples on our FACSAriaII - Aria3. Per Universal Precautions: All primary human samples will be considered BSL2 samples.

Prior to being able to run such samples, please complete the following questionnaire:

BSL2+ PI Questionnaire - To be filled out by the PI of any lab that wishes to sort BSL2 samples.

Our BSL2+ sorting policies can be found here. 
Please review this prior to sorting BSL2 samples with us.

If you have any questions about our new BSL2+ sorting capabilities, please don't hesistate to stop by or email us! 


Click on the links below to learn more about each of our instruments:

Aria 1 — FACSAriaIIu in hood
Aria 2 — FACSAriaII
Aria 3 — FACSAriaII in hood
LSRII
**COMING SOON: MACSQuant VYB


FORMS:

Biosafety Agreement – For all new users. Please complete and submit this form if you intend to use our core facility. This needs to be submitted to us before scheduling any time.

Human Sort Document – For anybody bringing live, tested, uninfected human samples (non-BSL2). You must bring this form every time you bring live human cells.
Anybody working with live human cells (non-BSL2 cell lines) MUST also bring documentation stating that the cells have been tested and found negative for HIV, HepB, and HepC and are otherwise non-infectious samples.

BSL2+ PI Questionnaire - To be filled out by the PI of anyone who wishes to sort BSL2 samples.

Sample/sort Drop Off Form - used during facility re-opening period to communicate experiment details 


Other useful information:


Introduction to Flow Cytometry – an excellent collection of training videos provided by BD Biosciences to teach you about flow cytometry
FlowBook - Intro to Flow - created by DeNovo Software, another great resource for learning about flow cytometry
Compensation Tutorial – courtesy of Dr. Mario Roederer, this website will teach you about compensation
Fluorescence Spectrum Viewer – use this to look for excitation/emission curves of many common fluorochromes
Fluorofinder - create multi-color panels with more confidence
New England Cytometry – stay up to date on all of the flow cytometry happenings in the New England area