Flow Cytometry Core

To provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area.

Personnel/Contact Information:

Director:
Director: Ronglih Liao, Ph.D.
Phone: (617) 525-4854
Fax: (617) 525-4868
Email: rliao@rics.bwh.harvard.edu

Other Staff:
Core Manager/Staff Scientist: Yi-Dong Lin, Ph.D. 
Phone: (617) 525-4861, (617) 525-4866 
Fax: (617) 525-4868
Email: ylin23@bwh.harvard.edu 

Facilities and Equipment:

Location of Core: 
77 Avenue Louis Pasteur, NRB 435E, Boston

Major Equipment:  
BD FACSAria Multicolor High Speed Sorter
BD Accuri C6 4-color Analyzer
Cytek DxP12 10-color Analyzer

Getting Started:

For more information on the core, please visit the website at:
http://bwhbri.partners.org/BWH_Flow_Cytometry_Core/Home_page.asp

Online sign-up:
Please register first with Yiling Qiu at yqiu@partners.org.

Sample Preparation:

What samples should I bring?

  • All users must always bring single stained compensation samples each stained with one color AND one unstained tube. Cell suspensions should be passed through a 35um nylon mesh filter (BD Biosciences part # 352235) right before sorting.
  • Don’t have enough cells for compensation?User can purchase compensation beads from BD BD CompBeads 552845 ‘Anti-Rat/Hamster’ for mouse samples BD CompBeads 552843 ‘Anti-Mouse’ for almost all human samples. 

Departmental Web Link:

http://bwhbri.partners.org/BWH_Flow_Cytometry_Core/Home_page.asp

Revised on 2018-01-04 19:41:11 UTC