- Cardiovascular Flow Cytometry Core
Flow Cytometry Core
To provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area.
Director: Ronglih Liao, Ph.D.
Manager, BWH flow cytometry core:
Grigoriy Losyev - Manager, BWH flow cytometry core
Administrator, BWH flow cytometry core:
Location of Core:
77 Avenue Louis Pasteur, NRB 435E, Boston
BD Accuri C6 4-color Analyzer
Cytek DxP12 10-color Analyzer
For more information on the core, please visit the website here
Please register first with Grigoriy Losyev at GLOSYEV@PARTNERS.ORG
What samples should I bring?
- All users must always bring single stained compensation samples each stained with one color AND one unstained tube. Cell suspensions should be passed through a 35um nylon mesh filter (BD Biosciences part # 352235) right before sorting.
- Don’t have enough cells for compensation?User can purchase compensation beads from BD BD CompBeads 552845 ‘Anti-Rat/Hamster’ for mouse samples BD CompBeads 552843 ‘Anti-Mouse’ for almost all human samples.