Flow Cytometry Core


Due to cuts in BWH support the Flow Core will close on July 31, 2025. We apologize for the inconvenience. Thank you for your continued support over the years, it has been our pleasure to work with you.


***************************************************************************************************************


To provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area.

Personnel/Contact Information:

Director:
Jane Leopold, MD
617-525-4846

Manager, BWH flow cytometry core:
Grigoriy Losyev
617-525-4748
GLOSYEV@PARTNERS.ORG

Administrator, BWH flow cytometry core:
Rick Bowman
617-525-4858
fbowman@bwh.harvard.edu

Location of Core: 
77 Avenue Louis Pasteur, NRB 435E, Boston

Major Equipment: 

BD Accuri C6 4-color Analyzer -- https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-cell-sorters/facsaria-fusion

CytoFLEX S V4-B2-Y4-R3 Flow Cytometer (13 Detectors, 4 Lasers) -- https://www.beckman.com/flow-cytometry/research-flow-cytometers/cytoflex-s/c09766

Cytek DxP12 10-color Analyzer

FY25 (effective 10/1/24) prices as follows:

Services Internal External NonProfit  External Industry
DxP11 Analyzer $               80.00   $             115.20  $             127.20
BD Aria Sorter Full Service $             110.00   $             158.20
  $             174.90
Cytoflex A/B $               80.00   $             115.20   $             127.20 


Getting Started:

For more information on the core, please visit the website here

Online sign-up:
Please register first with Grigoriy Losyev at GLOSYEV@PARTNERS.ORG

Sample Preparation:

What samples should I bring?

  • All users must always bring single stained compensation samples each stained with one color AND one unstained tube. Cell suspensions should be passed through a 35um nylon mesh filter (BD Biosciences part # 352235) right before sorting.
  • Don’t have enough cells for compensation?User can purchase compensation beads from BD BD CompBeads 552845 ‘Anti-Rat/Hamster’ for mouse samples BD CompBeads 552843 ‘Anti-Mouse’ for almost all human samples.