Mass General Brigham Center of Excellence for Molecular Imaging

 

Mass General Brigham Center of Excellence for Molecular Imaging is a core facility that provides microscopy training, state of the art imaging solutions, image processing and analysis services, and consultation services for researchers at Mass General Brigham Hospitals and neighboring institutes in Boston. The Core is located on the 4th floor of the CNY Building 149 in the Navy Yard, and is directed by Roy J. Soberman, M.D.

 

Available imaging techniques at the core:

 

  1. Bright Field Microscopy:
    Nikon Sora,  Nikon spinning disc 2,  Nikon N-STORM, Nikon Multiphoton 
  2. Epi Fluorescence Microscopy:
    Nikon Sora,  Nikon spinning disc 2,  Nikon N-STORM, Nikon Multiphoton 
  3. Confocal microscopy:
    Nikon Sora,  Nikon spinning disc 2,  Nikon Multiphoton 
  4. Super-resolution microscopy:
    Nikon Sora (down to 100nm),  Nikon N-STORM (down to 20nm, 10nm with nanobodies), Nikon Multiphoton (using the NSPARC addon, down to 100nm)
  5. Total Internal Reflection Fluorescence microscopy (TIRF):
    Nikon N-STORM
  6. Live cell imaging:
    Nikon Sora,  Nikon spinning disc 2
  7. Second Harmonic Generation SHG microscopy:
    Nikon Multiphoton 
  8. Fluorescence Spectroscopy (FLIM, FRET)
    Nikon Multiphoton
  9. Optical photomanipulation (photoablation, photodamage, optogenetics)
    Nikon Sora
  10. Live animal imaging
    Nikon Multiphoton (2 colors, red and green)
  11. Organoids, thick tissue (cleared and non-cleared)
    Nikon Sora (up to 100µm), Nikon Multiphoton (up to 500µm depending on the tissue type)
  12. NAD(P)H and FAD for determining the cellular status of energy metabolism
    Nikon Multiphoton

If you are intrested in using the core equipment, contact Mohammed Mahamdeh (mmahamdeh@mgh.harvard.edu) or Angela Schmider (aschmider@mgb.org).