Molecular Imaging Core
The Molecular Imaging Core at MGH provides services to investigators from MGH, BWH/Partners, and Harvard-affiliated institutions in the areas of molecular imaging of protein-protein interactions, and protein interactions with nucleic acids. Services are also available to other academic institutions and industry scientists.
The Core is located on the 4th floor of the CNY Building 149 in the Navy Yard, and is directed by Roy J. Soberman, M.D. The Core will provide full training of investigators and staff personnel, as well as complete performance of the technical aspects of all procedures if requested. Current services include Fluorescence Lifetime Imaging Microscopy (FLIM), time-lapse microscopy, and perfect focus/conventional immunofluorescence microscopy. Data storage and analysis, including FLIM data, is provided. The core provides a unique and important resource for members of the scientific community as they approach a variety of research problems that call for imaging/microscopy approaches to study protein interactions.
FLIM Process:
1. Consultation to determine experimental plan
Request a consultation under the Services tab of the Core website. Consultations usually last 60-90 minutes.
2. First time training to use the FLIM System
Request training on the Equipment page of the Core website.
Initial training and imaging of samples will last approximately 4 hours.
3. Self-service use of the FLIM System will be granted after training at the discretion of the core director in consultation with core technicians
Self-scheduling is available on the Equipment page of the Core website.
FLIM Systems are scheduled in 4-hour blocks, with 2 openings per day.
Please schedule 9am-1pm or 1pm-5pm.
4. Image analysis at Data Analysis Workstations
Self-scheduling is available on the Equipment page of the Core website.
Analysis takes approximately 5-10 hours per experiment.
We request that you schedule your time on our systems at least one week in advance when at all possible
Cancellation Policy: Notify Angela Schmider (aschmider@partners.org) and Selena Kim (Jkim136@mgh.harvard.edu) at least 24 hours in advance of your scheduled time. Cancellations less than 24 hours in advance will be charged for scheduled time.
