Physiological NMR Laboratory Core
The Physiological NMR Core currently provides the following services:
1. Simultaneously measure high energy Simultaneously measure high energy phosphates, inorganic phosphate, intracellular pH, Glucose uptake rate, ATP and phosphocreatine (PCr) synthesis rates in perfused organs (hearts, livers and skeletal muscles, etc) with 31P-NMR spectrometry
2. Simultaneously measure cardiac function with cardiac energetics in Langendorff system with 31P-NMR spectroscopy
3. Track substrate utilization/metabolism with 13C-NMR spectroscopy
4. Determine of intracellular Na content with 23Na-NMR spectroscopy.
5. Simultaneously measure following 27 metabolites of cellular redox and energy state in a single sample with HPLC ATP/ADP/AMP/Adenosine, Xanthine/Hypothanxine/Inosine/IMP, GTP/GDP/GMP, CTP/CDP/CMP, UTP/UDP/UMP, Malonyl-CoA/Acetyl-CoA/CoASH, NAD/NADH, NADP/NADPH, GSH/GSSG/Ascorbic Acid
Please note new FY '24 fees:
| Services | Internal | External NonProfit | External Industry |
| NMR User Assisted (per day) | $ 825.00 | $ 1,188.00 | $ 1,311.75 |
| NMR Full Service (per day) | $ 1,650.00 | $ 2,376.00 | $ 2,623.50 |
| NMR User Assisted Additional Hour | $ 150.00 | $ 216.00 | $ 238.50 |
| NMR Full Service Additional Hour | $ 250.00 | $ 360.00 | $ 397.50 |
| Biochemical Analysis (per sample) | $ 100.00 | $ 144.00 | $ 159.00 |
| Sample Preparation for Biochemical Analysis (per hour) | $ 100.00 | $ 144.00 | $ 159.00 |
| Data Analysis | $ 170.00 | $ 244.80 | $ 270.30 |
| Langendorff Perfusion | $ 125.00 | $ 180.00 | $ 198.75 |
Personnel/Contact Information:
Director: Jane Leopold, MD
Phone: (617) 525-4846
Email: jleopold@bwh.harvard.edu
Associate Director: Huamei He
Phone: (617) 525-5072
Email: hhe3@bwh.harvard.edu
Facilities and Equipment:
Location of Core:
221 Longwood Ave, BLI 242
Major Equipment:
9.4T Actively shielded AS400 Oxford Magnet
Waters 1525 Binary HPLC Pumps/DuallAbsorbance Detector
How to Get started?
To schedule a service please contact Associate Director:
Huamei He
Phone: (617) 525-5072
Email: hhe3@bwh.harvard.edu
