Physiological NMR Laboratory Core


The Physiological NMR Core currently provides the following services:

1. Simultaneously measure high energy Simultaneously measure high energy phosphates, inorganic phosphate, intracellular pH, Glucose uptake rate, ATP and phosphocreatine (PCr) synthesis rates in perfused organs (hearts, livers and skeletal muscles, etc) with 31P-NMR spectrometry

2. Simultaneously measure cardiac function with cardiac energetics in Langendorff system with 31P-NMR spectroscopy

3. Track substrate utilization/metabolism with 13C-NMR spectroscopy

4. Determine of intracellular Na content with 23Na-NMR spectroscopy.

5. Simultaneously measure following 27 metabolites of cellular redox and energy state in a single sample with HPLC ATP/ADP/AMP/Adenosine, Xanthine/Hypothanxine/Inosine/IMP, GTP/GDP/GMP, CTP/CDP/CMP, UTP/UDP/UMP, Malonyl-CoA/Acetyl-CoA/CoASH, NAD/NADH, NADP/NADPH, GSH/GSSG/Ascorbic Acid


Personnel/Contact Information:

Director: Ronglih Liao, Ph.D.
Phone: (617) 525-4854
Email: rliao@rics.bwh.harvard.edu

Associate Director: Huamei He

Phone: (617) 525-5072
Email: hhe3@bwh.harvard.edu


Facilities and Equipment:

Location of Core:
221 Longwood Ave, BLI 242

Major Equipment:
9.4T Actively shielded AS400 Oxford Magnet

Waters 1525 Binary HPLC Pumps/DuallAbsorbance Detector


How to Get started?

To schedule a service please contact Associate Director:

Huamei He
Phone: (617) 525-5072
Email: hhe3@bwh.harvard.edu

Or Huamei.He@gmail.com


Revised on 2018-06-12 14:27:10 UTC