- Viral Vector Core
Guide to non-MVC plasmid construction
| Viral vector submitted | Genetic elements |
|---|---|
| AAV vectors | ITR, promoter(s), transgene(s), polyA signal, any other genetic elements |
| Lentiviral vectors | 5’LTR, status of 3’LTR (deleted of all enhancer elements or not), cPPT, promoter(s), transgene(s), WPRE |
| Retroviral vectors | 5’LTR, status of 3’LTR (deleted of all enhancer elements or not), promoter(s), transgene(s), WPRE |
If possible, we recommend you include a fluorescent reporter protein in your plasmid. This will help us troubleshoot any problems that may arise during virus production, and will enable you to gauge the efficiency of transduction in your experiments.
Revised on 2017-07-10 18:46:29 UTC
