Hepatocytes are the most abundant type of cell in the human liver, making up an estimated 80% of the liver mass. They contain enzymes that are involved in phase I and phase II metabolism which are crucial to drug-drug interaction, metabolic stability and drug toxicity studies. The Cell Resource Core (CRC) isolates human hepatocytes from both whole livers and resected tissue. Hepatocyte isolations require extensive technical knowledge, and our many years of experience can assure the best quality primary hepatocytes on the market. These high-quality cells are ideal for metabolism, ADME-toxicity studies and many other applications. All cells are cultured at our facility as a reference to ensure the quality of the hepatocytes. Fresh human hepatocytes have a viability of >85% and are confluent in plate culture. Fresh cells can be prepared in suspension or plated to meet your requirements and can be shipped to you as soon as possible.

Our top-of-the-line isolations and thoroughly optimized cryopreservation process ensures the highest quality cryopreserved hepatocytes. Cryopreserved rat hepatocytes are provided with characterization of post-thaw viability and cell yield, attachment and basic metabolic function (urea, albumin production).

Mouse hepatocytes are isolated upon request with a viability of 90 ± 5%. Standard isolations are from CD1 mice and other strains can be requested.

Knockout Mouse- Hepatocytes isolated from knockout mice offer an ideal in vitro platform for investigation of your target pathway. Hepatocyte quality of custom isolations is often impacted by the knockout model, which our technicians are skilled in troubleshooting and optimizing. Please inquire about setting up an optimization pilot with us to provide high-quality hepatocytes from your knockout animals.

Additional charge for materials needed to Cryopreserved human hepatocytes. Cryopreserved hepatocytes offer several advantages such as vial–to–vial similarity and on-demand availability. This allows you to time your experiments at your convenience while minimizing experimental variability.

Isolated from a variety of donors, our inventory offers high-quality cells provided with a characterization of post-thaw viability and cell yield, attachment and basic metabolic function (urea, albumin production). Phase I (CYP450) drug metabolizing enzyme activities can be provided on request.

We offer comprehensive training in both microsurgery and cell culture techniques at our fully equipped facility. Our state-of-the-art surgical microscope and highly skilled microsurgical staff enable us to provide hands-on training and support for a wide range of surgical procedures, including organ, blood, and tissue procurement; soft tissue surgeries; device implantation; and vascular and non-vascular catheterization. We also offer guidance and customized protocols for organ transplantation—please inquire for more details.

In addition, we provide cell culture training with access to BSL-2 certified cell culture hoods and incubators. Our program is designed to support researchers at all levels, ensuring safe and effective handling of cell cultures in a controlled environment.

We provide high quality DMEM for cell culture.

Williams E-based medium optimized for use in primary hepatocyte cultures.

1 liter of solution that is used to recover organs during perfusion.

Our lab has developed and perfected machine organ perfusion of internal and external organs from a variety of species including rat, mouse and Non-Human primates. Furthermore, we specialize in a variety of perfusion temperature profiles including, normothermic, subnormal-thermic, and hypothermic perfusions.

Machine perfusion of human and swine organs for up to 12 hours.

Access to our small organ perfusion systems including Siemens machines, Piccolos, standard perfusion supplies, and setup and cleanup by a technician.

Access to our large organ perfusion systems including Siemens machines, Piccolos, standard perfusion supplies, and setup and cleanup by a technician.