Seahorse Core

The Seahorse Core supports the use of an XFe24 Extracellular Flux Analyzer from Seahorse Biosciences to perform sensitive measurements of oxygen consumption rate and extracellular acidification rate in cultured cells, tissues, or model organisms in a 24-well format.

Contact Information

Director: William Oldham, M.D., Ph.D.
Phone: 617-525-4834
Email: woldham@bwh.harvard.edu

Administrator: Rick Bowman
Phone: 617-525-4858
Email: fbowman@bwh.harvard.edu

Core email: BWHSeahorseCore@partners.org

Facilites and Equipment

Location: Harvard New Research Building 631, 77 Avenue Louis Pasteur, Boston

Hours: M-F, 8-5; Two assays run per day (morning and afternoon).

Equipment: XFe24 Extracellular Flux Analyzer, Prep Station

Services

  1. Basic Assay
    – core provides assay plate and assay cartridge
    – user provides samples in the assay plate, assay medium, and injection reagents
  2. Glyco Stress Assay
    – core provides assay plate, assay cartridge, Seahorse medium with supplements, and Seahorse reagents
    – user provides samples in the assay plate
  3. Mito Stress Assay
    – core provides assay plate, assay cartridge, Seahorse medium with supplements, and reagents
    – user provides samples in the assay plate

All services include use of the Seahorse Prep Station liquid handling system and non-CO2 incubator, cartridge hydration on the day prior to the assay, and technical assistance. Users are responsible for preparing media and injection reagents, performing media exchanges, loading the assay cartridges, running the assay, and analyzing the data. All new users require training.

User Fees

ServicesPHSAcademicIndustry
Basic Assay$368$530$710
Glyco Stress Assay $529$762$1020
Mito Stress Assay$473$681$910
Training$65/h$94/h$130/h

Getting Started

Navigate to https://researchcores.partners.org/ and create a user account based on the instructions in the quick guide (see link on the Research Cores Home Page). Provide the fund information that will be used to pay for services.

Complete the User Information Form and email it to the core at BWHSeahorseCore@partners.org.

Revised on 2017-04-10 12:04:16 UTC