Seahorse Core
The Seahorse Core falls under the Cardiovascular Flow Core and supports the use of an XFe24 Extracellular Flux Analyzer from Seahorse Biosciences to perform sensitive measurements of oxygen consumption rate and extracellular acidification rate in cultured cells, tissues, or model organisms in a 24-well format.
Contact Information
Director: William Oldham, M.D., Ph.D.
Phone: 617-525-4834
Email: woldham@bwh.harvard.edu
Administrator: Rick Bowman
Phone: 617-525-4858
Email: fbowman@bwh.harvard.edu
Facilites and Equipment
Location: Harvard New Research Building 631, 77 Avenue Louis Pasteur, Boston
Hours: M-F, 8-5; Two assays run per day (morning and afternoon).
Equipment: XFe24 Extracellular Flux Analyzer, Prep Station
Services
- Basic Assay – core provides assay plate and assay cartridge – user provides samples in the assay plate, assay medium, and injection reagents
- Glyco Stress Assay – core provides assay plate, assay cartridge, Seahorse medium with supplements, and Seahorse reagents – user provides samples in the assay plate. BY APPOINTMENT ONLY.
- Mito Stress Assay – core provides assay plate, assay cartridge, Seahorse medium with supplements, and reagents – user provides samples in the assay plate
All services include use of the Seahorse Prep Station liquid handling system and non-CO2 incubator, cartridge hydration on the day prior to the assay, and technical assistance. Users are responsible for preparing media and injection reagents, performing media exchanges, loading the assay cartridges, running the assay, and analyzing the data. All new users require training.
User Fees
Our new fees for FY24 will go into effect on 10/1/23 as follows:
| Services | PHS | Academic | Industry |
|---|---|---|---|
| Seahorse Test without Suplies (Basic Assay) | $425.00 | $612.00 | $675.75 |
| Mito Stress Assay with Supplies |
$540.00 | $777.60 | $858.60 |
| Cartridge | $90.00 | $129.60 | $143.10 |
| Training (minimum 3 hours) |
$125.00/hr | $180.00/hr | $198.75/hr |
Acknowledging Core Support
Please acknowledge the core facility in all publications, posters, and presentations. When appropriate, also acknowledge core staff for technical assistance with experiments or data analysis and consider their inclusion as authors when their scientific contributions merit this recognition. Please include the following statement in the acknowledgement section of manuscripts:
Cell bioenergetic analysis was performed in collaboration with the Seahorse Core in the Department of Medicine at Brigham and Women’s Hospital.
Getting Started
Navigate to https://researchcores.partners.org/ and create a user account based on the instructions in the quick guide (see link on the Research Cores Home Page). Provide the fund information that will be used to pay for services.
