The Antibody Core Facility provides commonly used monoclonal antibodies to the BWH research community. The antibodies are available in convenient 500µg aliquots at a cost substantially lower than that of commercial vendors.
The core generates clinically approved compounds for PET scans, research tracers for human use, preclinical tracers, novel tracers as requested by investigators and offer small animal imaging services such as microPET/CT and microSPECT.
The core provides high quality, reproducible measures of bone mineral density (BMD) and body composition by dual energy x-ray absorptiometry (DXA).
The core provides state-of-the-art high-quality research assays, especially useful for clinical research, to the investigators at reduced costs. The list of assays offered by the core may found in the core website.
The facility is equipped with, Olympus FV1200 confocal microscope (without multi-photon capability) consisting of an Argon 458/477/488/514 nm laser, a Green HeNE 543 nm, a Red HeNe 633 nm laser, and a Coherent ULTRA near infrared tunable Titanium Sapphire laser (690 - 1040 nm). The systems allow a range of experiments with fixed or live samples with time lapse function available on all systems making it possible to perform experiments over time courses in 5 different channels. Investigators with expertise in mammalian cells, yeast cells, Drosophila and Zebra fish embryos and ovaries, bacteria, and other tissues are available to help the users.
Provides zebra fish husbandry and help with procedures.
Provides small animal husbandry and help with procedures.
Provides access to over 3 million IRB approved frozen biosamples from Nurses Health studyI and II, Physicians Health Study I, Healthcare Professionals Follow-up Study and Growing-Up Today Study.
The Center for Advanced Molecular Diagnostics (CAMD) is a CLIA-certified Clinical Molecular Diagnostics Laboratory. Our mission is to provide high quality innovative molecular genetic diagnostic services to support the patient care missions of clinicians and clinical researchers.
The Core provides cell sorting and flow cytometry analysis services to the research community at the Brigham and WomenŠ—Ès Hospital (BWH) and the Longwood Medical area.
The Cardiovascular Physiology Core provides cutting edge preclinical research tools including surgery, non invasive rodent and fish imaging and multiplexed ex vivo and in vivo physiologic measurements, including blood pressure. It is a full service preclinical core serving the Partners research community, and its academic and industrial partners.
This Core provides flow cytometry cell sorting services and droplet-based single cell multiomics.
This core provides research investigators with experienced research staff to assist in the start-up, implementation, and close out of clinical research studies. Services provided include Biostatistics, Nursing and Nutrition, Outpatient services and Research coordination.
The goal of the core is to serve the computing needs of the epidemiology community of the Channing Laboratory and beyond. In that vein, the Core provides fast access to our server for data management and analysis and storage for large data sets especially for whole genome genetic studies.
The Chronobiology Core provides a central resource for trained personnel specialized in Chronobiology procedures, available to Investigators on an hourly basis.
The Circulating Tumor Cell (CTC) core provides users with FDA-approved method for the isolation and quantitation of circulating tumor cells from peripheral blood samples of patients with metastatic cancers.
The facility is equipped with, Nikon TE200-U inverted microscope with Nomarski optics, epifluorescence and scanning confocal system. A range of experiments with fixed or live samples with time lapse function and fluoresecence recovery after photobleaching (FRAP) are available.
Provides clinical samples or consented patient samples to research purposes.
The mission of the Cardiovascular, Diabetes, and Metabolic Disorders (CVDM) Research Center is to foster and stimulate collaborative research and technological innovation that will lead to groundbreaking and effective approaches for the diagnosis, prevention and treatment of cardiovascular diseases, diabetes, and obesity. The core is a repository of human myocardial, vascular, and stromal tissues.
The core offers conventional karyotyping and fluorescent in situ hybridization of both human and rodent samples and array based comparative genome hybridization.
The CoreÎ¾provides flexible, high-quality, high-throughput single nucleotide polymorphisms (SNP) genotyping to the research community. Its mission is to provide services to researchers conducting molecular analyses of germline and somatic DNA collected as part of a wide range of investigations into the molecular epidemiology of cancer, including Genome Wide Association Studies (GWAS).
The BWH Emergency Medicine Research Core provides research investigators with assistance in the implementation of clinical research studies, especially in the emergency department. This includes general research coordination (screening, consenting, performing study procedures, data entry), project management (IRB and regulatory documents maintenance), basic data service (REDCap development and maintenance, data entry). We can also provide Qualified Bilingual research staff who can interact with Spanish-speaking research participants).
GENOMICS INSTRUMENTS: High-throughput DNA and RNA sequencing As part of the service that our NeuroTech Studio provides, our customers can access the brand new Illumina NextSeq 550 sequencing machine. You can choose either to upload the data to Illumina’s cloud storage or save it to the designated space on Partners’ ERISone high-performance computing cluster. For later, we will take care of your data with seamless service, from data management, data backup, data QC, downstream NGS analysis, till the final uploading to GEO/dbGap before your publication. It’s an affordable, secured, and customized service that both you and your data deserve. Single-cell Genomics We also provide a single-cell genomics platform: 10x Genomics CHROMIUM CONTROLLER. You can use it for Single Cell CNV, Single Cell Gene Expression, Single Cell Immune Profiling, and Single Cell ATAC. Analysis Service At this stage, the program will primarily provide NGS data analysis service, including but not limited to: - Data management (storage/backup, meta-table management, GEO/dbGap submission), - RNA-seq analysis (from raw sequencing data to QC, to normalized gene expression table, group comparison, pathway analysis, and interactive reporting and visualization of the results), - Small RNA-seq analysis (e.g. miRNAs, circRNAs, piRNAs, eRNAs, etc.), - Single-cell omics analysis (10x Genomics pipeline, clustering, trajectory analysis, spatial transcriptomics, etc.), - ChIP-seq analysis (e.g. QC, peak calling, differential binding, UCSC Genome Browser visualization, etc.) - Variant calling from genotyping/WGS/WES data, and - Other next-generation sequencing (NGS) data analysis (ATAC-seq, CAGE, ChIA-PET, Hi-C, etc.)
The HSDRC provides access to fresh and banked human skin specimens and cutting edge techniques for analysis of human skin including CyTOF mass cytometry, multiplex immunofluorescence microscopy, T cell receptor sequencing, and NanoString gene expression analysis.
The ID Retrovirology Core processes samples from HIV- and HCV-infected patients participating in clinical research studies. Services include plasma and serum separation, peripheral blood Cncermononuclear cell isolation and cryopreservation, and preparation of cell pellets.
Breaking down barriers to translational research is the key to finding new approaches to inflammatory arthritis in adults and children.
This research resource core will organize, label, maintain, and distribute rare-earth metal labeled antibodies for CyTOF mass cytometry.
The core provides breeding and husbandry of gnotobiotic mice and assists investigators with analyses regarding effects of metabolic, immunologic and other physiologic effects following host colonization commensal and/or pathogenic microbes. The core also provides consults on microbiome and bioinformatics.
The core offers Comprehensive Laboratory Animal Monitoring System (CLAMS) studies to noninvasively measure a variety of calorimetric and metabolic data in mice. The calorimetric data recorded by the system includes oxygen consumption and carbon dioxide production values, from which both heat values and respiratory exchange ratios (RER) can be calculated.
The core provides the necessary expertise and services to analyze variations in individual genome that translates into medical decisions, practices, interventions tailored to the individual patient (or a small cohort) based on their predicted response or risk of disease.
The BWH NeuroTechnology Studio provides access to advanced instrumentation for optical microscopy, genomics, metabolic imaging and other technologies, including image analysis software. We also provide expert support and training for new and experienced users. Our primary focus is neuroscience research but we also welcome users from other fields.
Center for Integrated Healthcare Data Research
The Integrated Analytics core has been established by the division of Pharmacoepidemiology and Pharmacoeconomics to allocate computer programmer effort and closely related costs to federal and non-federal projects. The division of Pharmacoepidemiology and Pharmacoeconomics specializes in analyzing massive data sets related to prescription data use.
The core will serve as a resource for studies in cardiovascular medicine using NMR. The core provides tools to define the cardiac energetics (using 31P NMR), metabolism (using 13C NMR) and hemodynamic function (the isovolumic contractile performance) of isolated hearts.
Provides imaging service for research purposes.
The core houses an Agilent Seahorse XFe24 instrument and provides functional metabolic studies on live tissues and cells.
The Sleep & EEG Core within the Division of Sleep Medicine (DSM) provides an integrated infrastructure and knowledge base in support of research projects that use polysomnography (PSG), quantitative EEG analysis and related methodologies
The Core provides cell sorting and flow cytometry analysis services to the research community at the Brigham and Women's Hospital (BWH) and the Longwood Medical area.
The aims to establish the impact of genetic variation on the function of the human body. It is also a resource that provides a source of fresh and stored biological samples from healthy human subjects, as well as an electronic atlas that references the expression of genes to the variation found within those genes for research use.
Centralized repository of patient and normal tissue and blood samples.
The Translational Discovery Laboratory (Core) provides state of the art facility and trained staff for large animal experimentation with an emphasis on percutaneous cardiovascular technologies.